3D positioning and optically detected magnetic resonance of intracellular fluorescent nanodiamonds using a multi-plane microscope
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3D positioning and optically detected magnetic resonance of intracellular fluorescent nanodiamonds using a multi-plane microscope

22/02/2026 Frontiers Journals
Wide-field quantum sensing with fluorescent nanodiamonds (FNDs) in biological systems offers significant potential for understanding intracellular dynamics at the nanoscale. However, current wide-field quantum sensing methods are limited to 2D correlated measurements. 3D correlated quantum sensing remains challenging due to the inherent properties of wide-field microscopy. Here, we have developed a multi-plane wide-field microscope platform that achieves an imaging volume of 50 × 50 × 5 μm³. This is accomplished by simultaneously imaging eight focal planes at varying sample depths using a beam-splitting prism. By employing a Fourier-transform-based fluorescent particle positioning method, the platform attains lateral positioning precision of 9 nm and axial precision of 12 nm. Using this platform, we performed correlated 3D positioning of FNDs in mouse cardiomyocytes and conducted optically detected magnetic resonance on nitrogen-vacancy color centers within intracellular FNDs. Our results demonstrate the potential of this platform for single-particle tracking and highlight its capability to achieve correlated 3D quantum sensing.
The work entitled “3D positioning and optically detected magnetic resonance of intracellular fluorescent nanodiamonds using a multi-plane microscope” was published in Biophysics Reports (ahead of print in Feb. 2026).
DOI: 10.52601/bpr.2025.250012
https://www.biophysics-reports.org/article/doi/10.52601/bpr.2025.250012
Attached files
  • Image: Schematic diagram of multi-plane microscope. The model of the laser is Coherent Verdi G5. OBJ is the microscopic objective (Olympus LUCPLFLN40X).
22/02/2026 Frontiers Journals
Regions: Asia, China
Keywords: Science, Life Sciences

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