3D positioning and optically detected magnetic resonance of intracellular fluorescent nanodiamonds using a multi-plane microscope
en-GBde-DEes-ESfr-FR

3D positioning and optically detected magnetic resonance of intracellular fluorescent nanodiamonds using a multi-plane microscope

22.02.2026 Frontiers Journals
Wide-field quantum sensing with fluorescent nanodiamonds (FNDs) in biological systems offers significant potential for understanding intracellular dynamics at the nanoscale. However, current wide-field quantum sensing methods are limited to 2D correlated measurements. 3D correlated quantum sensing remains challenging due to the inherent properties of wide-field microscopy. Here, we have developed a multi-plane wide-field microscope platform that achieves an imaging volume of 50 × 50 × 5 μm³. This is accomplished by simultaneously imaging eight focal planes at varying sample depths using a beam-splitting prism. By employing a Fourier-transform-based fluorescent particle positioning method, the platform attains lateral positioning precision of 9 nm and axial precision of 12 nm. Using this platform, we performed correlated 3D positioning of FNDs in mouse cardiomyocytes and conducted optically detected magnetic resonance on nitrogen-vacancy color centers within intracellular FNDs. Our results demonstrate the potential of this platform for single-particle tracking and highlight its capability to achieve correlated 3D quantum sensing.
The work entitled “3D positioning and optically detected magnetic resonance of intracellular fluorescent nanodiamonds using a multi-plane microscope” was published in Biophysics Reports (ahead of print in Feb. 2026).
DOI: 10.52601/bpr.2025.250012
https://www.biophysics-reports.org/article/doi/10.52601/bpr.2025.250012
Angehängte Dokumente
  • Image: Schematic diagram of multi-plane microscope. The model of the laser is Coherent Verdi G5. OBJ is the microscopic objective (Olympus LUCPLFLN40X).
22.02.2026 Frontiers Journals
Regions: Asia, China
Keywords: Science, Life Sciences

Disclaimer: AlphaGalileo is not responsible for the accuracy of content posted to AlphaGalileo by contributing institutions or for the use of any information through the AlphaGalileo system.

Referenzen

We have used AlphaGalileo since its foundation but frankly we need it more than ever now to ensure our research news is heard across Europe, Asia and North America. As one of the UK’s leading research universities we want to continue to work with other outstanding researchers in Europe. AlphaGalileo helps us to continue to bring our research story to them and the rest of the world.
Peter Dunn, Director of Press and Media Relations at the University of Warwick
AlphaGalileo has helped us more than double our reach at SciDev.Net. The service has enabled our journalists around the world to reach the mainstream media with articles about the impact of science on people in low- and middle-income countries, leading to big increases in the number of SciDev.Net articles that have been republished.
Ben Deighton, SciDevNet
AlphaGalileo is a great source of global research news. I use it regularly.
Robert Lee Hotz, LA Times

Wir arbeiten eng zusammen mit...

  • e
  • The Research Council of Norway
  • SciDevNet
  • Swiss National Science Foundation
  • iesResearch
Copyright 2026 by DNN Corp Terms Of Use Privacy Statement