Effect of Chlorpyrifos on Spermatogenesis
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Effect of Chlorpyrifos on Spermatogenesis

01/07/2025 Compuscript Ltd

Chlorpyrifos (CPF) is a widely used organophosphorus pesticide known for its detrimental effects on human health. It enters the body through dermal contact, ingestion, or the food chain, leading to accumulation and metabolism. Its primary metabolite, 3,5,6-trichloro-2-pyridinol (TCP), can cause significant damage to multiple organs and systems.

Recent studies suggest that CPF has negative implications on male reproduction, as evidenced by decreased levels of serum testosterone, follicle-stimulating hormone, and luteinizing hormone, along with reduced sperm count and quality. However, its toxic effects and the specific mechanisms underlying the junctional function of Sertoli cells requires further investigation.
In a recent study, published in the Genes & Diseases journal, researchers at the Children's Hospital of Chongqing Medical University and the Second Affiliated Hospital of Chongqing Medical University demonstrated that CPF exposure induces spermatogenesis dysfunction by impairing blood-testis barrier (BTB) integrity, which is regulated by clockophagy-mediated ferroptosis.

Preliminary findings showed that CPF impairs spermatogenesis by compromising the BTB integrity in SD rats. In vitro results suggest that exposure to TCP disrupts junctional function in Sertoli cells, contributing to the loss of BTB integrity. RNA sequencing of Sertoli cells exposed to TCP, followed by KEGG analysis, identified DEGs primarily enriched in metabolic pathways, glycolysis, and ferroptosis. Further experimentation confirmed that TCP disrupts Sertoli cell junctional function by inducing ferroptosis.

Clockophagy is a newly identified type of selective autophagy that triggers ferroptosis by degrading ARNTL, a core circadian clock protein, and thereby causing instability of HIF-1α. Following TCP exposure, the protein expression of ARNTL, GPx4, and light chain3 beta (LC3B) II/I decreased with a concomitant increase in the protein levels of p62 and prolyl hydroxylase 1 (PHD1), suggesting that TCP exposure induces ferroptosis in Sertoli cells by activating clockophagy. Additionally, in vivo experiments further confirmed that CPF exposure induces ferroptosis in the testes via clockophagy.

The authors acknowledge that the study has certain limitations, such as: i) the use of higher CPF doses than those encountered in realistic human exposure scenarios; ii) the use of only pre-pubertal male rats (including rats of all age groups would provide a better understanding of the toxic effects of CPF on male reproduction); and iii) not investigating the roles of secretion and phagocytosis, both of which play crucial roles in spermatogenesis.

In conclusion, the findings of this study provide molecular insights into how CPF exposure induces male infertility by compromising the BTB integrity, highlighting the potential influence of clockophagy- mediated ferroptosis in spermatogenic dysfunction.

Reference

Title of the original paper: Chlorpyrifos induces spermatogenic dysfunction via ferroptosis in Sertoli cells

Journal: Genes & Diseases

Genes & Diseases is a journal for molecular and translational medicine. The journal primarily focuses on publishing investigations on the molecular bases and experimental therapeutics of human diseases. Publication formats include full length research article, review article, short communication, correspondence, perspectives, commentary, views on news, and research watch.

DOI: https://doi.org/10.1016/j.gendis.2025.101601

Funding Information:

National Natural Science Foundation of China (No. 81801521)
Chongqing Science and Technology Bureau of China (No. CSTB2022NSCQ-LZX0019, 2022ZDXM033)
Chongqing Municipal Education Commission of China (No. KJQN202200410)

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Genes & Diseases publishes rigorously peer-reviewed and high quality original articles and authoritative reviews that focus on the molecular bases of human diseases. Emphasis is placed on hypothesis-driven, mechanistic studies relevant to pathogenesis and/or experimental therapeutics of human diseases. The journal has worldwide authorship, and a broad scope in basic and translational biomedical research of molecular biology, molecular genetics, and cell biology, including but not limited to cell proliferation and apoptosis, signal transduction, stem cell biology, developmental biology, gene regulation and epigenetics, cancer biology, immunity and infection, neuroscience, disease-specific animal models, gene and cell-based therapies, and regenerative medicine.

Scopus CiteScore: 8.4 | Impact Factor: 9.4

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Fichiers joints
  • (A) The level of Fe2+ in the three different groups. (B) The level of malondialdehyde (MDA) in the three different groups. (C) The level of glutathione (GSH) in the three different groups. (D) The protein expression level of GPX4 in the three different groups. (E) The protein expression levels of markers of clockophagy and lysosomal function in the three different groups. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001; ns, not significant.
  • (A) The histological changes in the testes of rats in the three different groups. (B) The sperm deformation rate. (C) The number of sperm. (D) Immunofluorescence analysis of the expression of PLZF and Stra8 in the rat testes. (E) The protein expression levels of PLZF and Stra8 in the rat testes by western blot. ***P < 0.001 and ****P < 0.0001.
  • (A) The differentially expressed genes between the DMSO and TCP groups. (B) The results of the KEGG analysis. (C) The levels of Fe2+, malondialdehyde (MDA), and glutathione (GSH) in Sertoli cells in the four different groups. (D) The protein expression level of GPX4 after TCP exposure. (E) Reactive oxygen species (ROS) generation in the four different groups. (F) Calcein-AM/propidium iodine (PI) staining showed that ferrostatin-1 (Fer-1) treatment reversed the cell death induced by TCP exposure. (G) FerroOrange staining revealed that Fer-1 treatment decreased the level of ferrous ions induced by TCP exposure. (H) Fer-1 treatment reduced the level of lipid peroxidation induced by TCP exposure. (I) Fer-1 treatment reversed the decrease in the protein expression levels of blood-testis barrier-associated proteins after TCP exposure. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001; ns, not significant.
01/07/2025 Compuscript Ltd
Regions: Europe, Ireland, Asia, China
Keywords: Health, Medical, Science, Life Sciences

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