Bacterial motility is critical for niche exploration, competitive fitness, and host infection. Flagella and type IV pili (T4P) are two common motility systems in bacteria, mediating swimming in liquids and swarming or twitching on solid surfaces, respectively. Xanthomonas campestris pv. campestris (Xcc), an important phytopathogen within the genus Xanthomonas, possesses both a single polar flagellum and peritrichous T4P, making it a model organism for studying plant–microbe interactions. However, the regulatory mechanisms governing motility in xanthomonads and their contributions to host colonization remain poorly understood.
Although two-component system proteins containing tandem receiver domains are widespread in bacteria, their functions remain poorly understood. In this study, the authors characterized DrdR, a unique response regulator in Xcc that contains tandem receiver domains but lacks an output domain. They found that DrdR positively regulates swarming motility while negatively regulating swimming, modulates chemotactic responses to diverse attractants, and plays an important role during early infection. Co-immunoprecipitation and protein–protein interaction assays showed that DrdR physically interacts with the T4P motor proteins PilB and PilT. Enzymatic and genetic analyses further showed that DrdR enhances the ATPase activity of PilB/PilT to promote pilus extension and retraction, thereby controlling Xcc swarming. Bioinformatics analysis combined with site-directed mutagenesis indicated that one of the two REC domains in DrdR is a canonical functional receiver domain with an intact active pocket and conserved phosphorylation-site residues, whereas the other is a pseudo-receiver domain incapable of phospho-signal reception. Collectively, the authors proposed a working model in which, upon sensing plant-derived environmental signals, an as-yet-unidentified upstream histidine kinase activates DrdR. The subsequent increase in DrdR activity and abundance stimulates the ATPase activity of the pilus motor proteins, driving Xcc swarming on plant surfaces and ultimately enhancing bacterial colonization of leaf hydathodes.
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References
DOI
10.1002/mlf2.70092
Original Source URL
https://doi.org/10.1002/mlf2.70092
Funding information
This work was supported by the National Natural Science Foundation of China (32160036 and 32360649) and the Natural Science Foundation of Guangxi Zhuang Autonomous Region of China (2023GXNSFAA026394).
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mLife is an open access microbiology journal, sponsored by the Institute of Microbiology of the Chinese Academy of Sciences, in cooperation with the Chinese Society for Microbiology. The journal aims to publish novel and high-impact discoveries in a wide spectrum of disciplines in microbiology. mLife has been indexed by ESCI, PubMed, Scopus, DOAJ, CSCD, CAS, Google Scholar, etc. The 2025 Journal Citation Reports impact factor (released in 2026) is 6.7 (Q1/Microbiology).