Plants face constant threats from herbivores, prompting complex defense mechanisms. While grasses like rice and Arabidopsis have well-studied defense systems, woody plants like tea remain poorly understood. Previous research highlighted LRR-RLKs as key regulators of herbivore perception and defense initiation in grasses, but their roles in woody species were unclear. Tea plants, a vital economic crop, suffer significant losses from pests like the tea geometrid caterpillar. Preliminary studies suggested LRR-RLKs might be involved, but the mechanisms were unknown. Understanding these processes is critical for developing sustainable pest management strategies. Based on these challenges, there was a pressing need to explore how LRR-RLKs function in tea plants to unlock their defense potential.
Published (DOI: 10.1093/hr/uhae281) on October 2, 2024, in Horticulture Research, a study by researchers from the Tea Research Institute of the Chinese Academy of Agricultural Sciences identified two genes, CsLRR-RLK44 and CsLRR-RLK239, as critical for tea plant defense against herbivores. Using genomic and transcriptomic analyses, the team demonstrated that these genes activate defense pathways, including jasmonate signaling, to combat pests like the tea geometrid. The findings provide the first evidence of LRR-RLKs’ role in woody plants, bridging a knowledge gap between grasses and economically important tree species.
The study systematically identified 307 LRR-RLK genes in tea plants, narrowing focus to CsLRR-RLK44 and CsLRR-RLK239 due to their strong correlation with herbivore resistance. These genes, localized to the plasma membrane, responded intensely to herbivore-associated molecular patterns (HAMPs), suggesting their role as receptors. Unlike some LRR-RLKs, they did not form homodimers or heterodimers, hinting at unique regulatory mechanisms. Silencing these genes reduced tea plants’ resistance, confirming their importance. The researchers linked their activity to downstream defense pathways: CsLRR-RLK44 and CsLRR-RLK239 upregulated MPKs and WRKY transcription factors, which in turn boosted jasmonate production—a key hormone in pest resistance. Notably, jasmonate levels dropped in silenced plants, weakening defenses. The study’s highlight lies in its translational impact. By elucidating the signaling cascade from receptor activation to hormone production, it offers a blueprint for enhancing crop resilience. The team’s use of antisense oligonucleotides for gene silencing also showcases a practical tool for studying non-model plants like tea.
Dr. Meng Ye, co-corresponding author, emphasized the study’s significance: “Our work uncovers the conserved role of LRR-RLKs in woody plants, revealing how tea plants orchestrate defenses against pests. This opens doors to breeding or engineering crops with heightened resistance, reducing pesticide use.” Co-author Jianyan Huang added, “Understanding these pathways at the molecular level is a game-changer for sustainable agriculture, especially for high-value crops like tea.”
The findings pave the way for developing tea varieties with enhanced pest resistance through targeted gene editing or marker-assisted breeding. Farmers could adopt these varieties to minimize yield losses and chemical inputs, aligning with eco-friendly practices. Beyond tea, the study’s framework applies to other woody crops, such as coffee or fruit trees, where herbivore damage is costly. Future research could explore the ligands activating CsLRR-RLK44/239, enabling synthetic biology solutions like designer peptides to trigger defenses. Meanwhile, integrating these genes into agricultural models may optimize pest management strategies globally. By bridging fundamental science and practical applications, this study marks a leap toward sustainable crop protection.
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References
DOI
10.1093/hr/uhae281
Original Source URL
https://doi.org/10.1093/hr/uhae281
Funding information
We thank Prof. Dr. Xinchao Wang for kindly supplying experimental materials. This research was funded by National Natural Science Foundation of China (32372775), Central Public-interest Scientific Institution Basal Research Fund (Y2023PT03), the Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding–Tea Plant (2021C02067), and the Elite Youth Program of Chinese Academy of Agricultural Sciences.
About Horticulture Research
Horticulture Research is an open access journal of Nanjing Agricultural University and ranked number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2023. The journal is committed to publishing original research articles, reviews, perspectives, comments, correspondence articles and letters to the editor related to all major horticultural plants and disciplines, including biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.