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First reference sequence of a bread wheat chromosome revealed

18 July 2014 INRA-France

Scientists announced the publication of the first reference sequence[1] of the biggest bread wheat chromosome. Thanks to an international collaboration, coordinated by INRA jointly with CEA (Genoscope), CNRS and Université d’Evry in France, this major achievement will allow the identification of numerous genes of agronomic interest, and accelerate wheat improvement. Scientists estimate that following this approach, a full reference genome sequence can be produced within three years. Results are published in Science on July 18th, 2014.

Bread wheat (Triticum aestivum L.) is the most important crop in the world in terms of land use and is the staple food for over a third of the world population. Current bread wheat varieties are the result of continuous selection conducted by human for thousands of years. Wheat holds a complex genome[2] (polyploid) comprised of three sub-genomes; altogether it is five-fold bigger than the human genome. “Although this complexity makes wheat a good model for studying large polyploid genomes, deciphering its sequence represented a great methodological challenge that delayed the advances in wheat genomics and particularly the sequencing”, says Frédéric Choulet, main author of the article published in Science.

A worldwide first

In the framework of a project called 3BSEQ, funded by ANR (French National Research Agency) and France Agrimer, scientists from INRA, CEA (Genoscope), CNRS, Université d’Evry, and international partners, produced the first reference sequence of the bread wheat chromosome 3B. To achieve this, several next generation sequencing technologies were combined, specific bioinformatic tools were developed and thousands of molecular markers were used. The result is a single sequence of 774 million nucleotides, gathering 8,500 long DNA fragments ordered along the chromosome.

Scientists compared this sequence with genomic data from related species such as rice or sorghum, and they discovered that 35% of the 7,700 genes identified on chromosome 3B were located on other chromosomes in the other cereals. This suggests that these genes currently found on chromosome 3B are the result of massive genomic rearrangements which are relatively recent from an evolutionary point of view and specific to wheat.

Recent and specialized genes at chromosome ends

By analyzing the sequence, researchers discovered a marked partitioning of the chromosome with contrasted patterns of organization, expression level and evolution rate between the extremities and the central region. While the extremities are significantly enriched in genes related to adaptation of the plant, expressed at a generally low level and specifically in certain tissues or stages of development, the central region is mainly composed of conserved genes involved in the basic metabolism of the plant.

The higher level of gene density at the ends can be explained by recent duplications, an evolutionary force stronger in wheat than in the other cereals. Thus, the massive expansion of the wheat genome was accompanied by a structural and functional partitioning and by the acquisition of new genes with adaptive functions.

A unique resource for the improvement of wheat breeding

“The reference sequence of chromosome 3B is a unique resource worldwide and it offers new opportunities for the identification of important genes of agronomic interest”, comments Catherine Feuillet, who led the project. As an example, some genomic regions of chromosome 3B are involved in the resistance to pathogens, in the tolerance to drought, and in yield; the specific genes controlling these traits are currently being identified. “A better understanding of the bread wheat genome will have two major impacts: from a basic science point of view, it will help understanding mechanisms impacting its organization, functioning and evolution; it will also pave the way to the development of the necessary tools and methodologies to improve the efficiency of selection programs” adds Choulet.

Buoyed by this success, the International Wheat Genome Sequencing Consortium (IWGSC,, in which INRA holds a leadership position, aims to finish sequencing the twenty other chromosomes within three years. French scientists from INRA and CEA (Genoscope) have already begun the sequencing of 2 other chromosomes.

[1] A reference sequence represents the assembled and ordered sequence of a chromosome, in the present case the bread wheat chromosome 3B, which will be used for all future research on wheat genetics.

[2] The genome gathers all the chromosomes, and by extension all the genes of an individual.

Attached files

  • INRA/Hervé Cochard

  • Sequencing the wheat genome, interview with Frédéric Choulet

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